Connectors and Reactor Coils
The majority of FI and SI systems are built with tubing I.D. of 0.5 mm to 0.8 mm. There are many tubing materials available for construction of reactor coils and connection lines. Teflon and PEEK are the most frequently used polymers. Stainless steel is another material that has the advantage of heat conductivity, gas impermeability, and surface properties that minimize protein adsorption. Most polymer tubing is translucent and is often color coded, so that tubing I.D. can be identified at glance.
Connectors made of color-coded polymers are fitted with ferrules that are designed to grip tubing while the connector nut is being tightened. Since all FIA systems operate at low pressure, it is not necessary to use connectors designed for HPLC. It is, however, very important to use nuts, ferrules and fittings from a single manufacturer, as products from different sources are often incompatible, resulting in leaks.
1.4.9.
U-200M - 1/4-28 Flat Bottom Union (male), T-1522 - 1/16th" OD Teflon Tubing, 0.03" ID
P-200X - 1/4-28 Flangeless Ferrules for 1/16" OD Tubing
P-286X - 1/4-28 Flangeless Nuts for 1/16" OD Tubing, PPS
U-200F - 1/4-28 Flat Bottom Union (female), PEEK, 1/16th“
Teflon reactor coil
Heated reactor coil with temperature controller
